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Title:Razvoj metode za HPLC-analizo izbranih fenolnih spojin lesa
Authors:ID Poljanšek, Ida (Author)
ID Vek, Viljem (Author)
ID Oven, Primož (Author)
Files:.pdf PDF - Presentation file, download (532,08 KB)
 
URL URL - Presentation file, visit http://eprints.gozdis.si/1860/
 
Language:Slovenian
Typology:1.01 - Original Scientific Article
Organization:Logo SciVie - Slovenian Forestry Institute
Abstract:Preučevali smo vpliv sestave mobilne faze na ločitev enostavnih fenolnih spojin, kot so galna kislina, pirokatehol, floroglucinol, resorcinol, vanilin in flavonoida katehina s tekočinsko kromatografijo visoke ločljivosti (HPLC). Kot najbolj primerna metoda za ločitev komponent se je izkazala metoda gradientnega izpiranja s programiranim spreminjanjem sestave mobilne faze acetonitril in 2-odstotna vodna raztopina mravljinčne kisline od 2 : 98 do 25 : 75. Ločitev organskih spojin je bila učinkovitejša v mobilni fazi z manjšim deležem mravljinčne kisline. HPLC je primerna metoda za identifikacijo in kvantitativno analizo spojin v različnih tipih vzorcev na širokem področju verige gozdarstva, lesarstva in papirništva.
Keywords:les, ekstraktivi, fenolne spojine, katehin, kromatografija, HPLC, ločba
Year of publishing:2015
Number of pages:str. 11-17
Numbering:[Št.] 108
COBISS.SI-ID:4357030 New window
UDC:630*84:547.562.4
ISSN on article:2335-3112
URN:URN:NBN:SI:doc-ARHB6K2T
Note:Besedilo v slov.;
Publication date in DiRROS:12.07.2017
Views:513
Downloads:36
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Record is a part of a journal

Title:Acta silvae et ligni
Publisher:Gozdarski inštitut Slovenije, založba Silvae Slovenica, Biotehniška fakulteta, Oddelek za gozdarstvo in obnovljive gozdne vire, Biotehniška fakulteta, Oddelek za lesarstvo
ISSN:2335-3112
COBISS.SI-ID:266761216 New window

Secondary language

Language:English
Title:Development of the HPLC method for separation of some phenolic compounds of wood
Abstract:The most suitable composition of mobile phase for the separation of simple phenolic compounds such as gallic acid, pyrocatechol, phloroglucinol, resorcinol, vanillin and related compound catechin by the high performance liquid chromatography was investigated. The most appropriate developing mobile system for the separation of these organic compounds was acetonitrile and 2% water solution of formic acid with gradient ratio from 2.98 to 25:75. The best separation of the six organic compounds was achieved using mobile phase with lower content of formic acid. HPLC is a method of choice for qualitative and quantitative analysis compounds in various types of samples in the broad field of forestry-wood-pulp-paper chain.


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This document is a part of these collections:
  1. Acta Silvae et Ligni

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